STUDIES ON RHEUMATOID FACTOR

Studies on the isolation of rheumatoid factor.

The need for the isolation of rheumatoid factor in a high state of purity and in sufficient quantity for antiserum production has led to an appraisal of the various purification methods available. Ziff, Brown, Lospalluto, Badin, and McEwen (1956) demonstrated that the sensitized sheep cell agglutinating activity of rheumatoid serum was precipitated in the euglobulin fraction, and Svartz and Sch...

Studies on rheumatoid agglutination factor in myocardial infarction.

portion of 19S and 4S fractions were increased in the positive cases of R.A. test. R.A. test of only the 19S component was positive in all cases. It is considered that there is an outflow of the necrotizing substance of the infarcted tissue into the circulatory blood and the appearance of a serum factor similar to an antibody against an antigen. Therefore, it is speculated that a cardiac antige...

Studies on the isolation and characterization of the "rheumatoid factor".

Chromatographic Studies of the Rheumatoid Factor

Serum and serum fractions from patients with rheumatoid arthritis have been subjected to anion exchange chromatography on diethylaminoethyl cellulose. The rheumatoid factor activity was clearly separated from the bulk of the gamma globulin by virtue of the anomalous behavior of macroglobulins on this column. Purification of rheumatoid factor by precipitation with fraction II, resolution in 4 M ...

HLA studies in IgM rheumatoid-factor-positive arthritis of childhood.

The clinical course of children with IgM rheumatoid-factor-positive chronic arthritis closely resembles that of seropositive rheumatoid disease in adults. The frequency of HLA DR4 is known to be increased in adults with seropositive rheumatoid arthritis, but the first major report on childhood arthritis did not suggest a correlation, though only 8 seropositive cases were included. Fifty-two chi...